Chem. Pharm. Bull. 53(7) 856—860 (2005)

نویسندگان

  • Zhongzhen ZHAO
  • Jia LU
  • Kelvin LEUNG
  • Chi Leung CHAN
  • Zhi - Hong
چکیده

cablin (BLANCO) BENTH. (Family Labiatae), commonly known as “Guang-Huo-Xiang” or Cablin Patchouli. It is a Chinese Materia Medica traditionally used for the treatment of common cold, nausea and diarrhea. Patchouli oil, the volatile oil of P. cablin, has been widely used in the cosmetic and oral hygiene industries to scent perfumes, flavor toothpaste, etc. Modern researches have repeatedly demonstrated various pharmacological activities of this oil including anti-emetic properties, trypanocidal activities, anti-bacterial, anti-fungal and Ca antagonist activities. It was reported that P. cablin contains sesquiterpenes cytotoxic chalcones, antimutagenic flavones. Patchoulic alcohol (Chart 1) is a major active ingredient of Herba Pogostemonis and is the most odor-intensive component of patchoulic oil. Therefore, the patchoulic alcohol content has been widely used as an indicator for the quality assessment of Herba Pogostemonis or dried P. cablin. Determination of patchoulic alcohol content, however, is not straightforward owing to the inherent chemical complexity of Herba Pogostemonis. Conventional methods always failed to give reliable results. For example, GC equipped with packed column may encounter difficulties in achieving satisfactory resolution. Capillary GC interfaced with Flame Ionization Detector (GC/FID) did not give steady outputs as a result of interfering GC peaks. To overcome these difficulties, we have developed a GC/MS/MS method to determine the patchoulic alcohol content in the whole herbs, stems and leaves of Herba Pogostemonis. All samples were freeze-dried in advance and then extracted with liquid carbon dioxide by supercritical fluid extraction to minimize the loss or decomposition of the active ingredient in the herbs. Supercritical fluid extraction is particularly useful for the extraction of volatile oils and thermally liable compounds. It has the advantage of extraction at a relatively low temperature, negligible solvent residue and relatively high extraction efficiency. Quantitative analysis was conducted by measuring the abundance of product ions of patchoulic alcohol in the MSMS spectrum. The identity of target compound was confirmed by full-scan mode and MS/MS scan mode by matching the retention time with an authentic standard. The enhanced selectivity of GC/MS/MS can eliminate the possible chromatographic interference occurred in GC/FID analysis. The minimized chromatographic interferences observed in the present study indicated suitability of the developed method for quality evaluation and quality control of Herba Pogostemonis.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Chem. Pharm. Bull. 53(6) 714—716 (2005)

tion, stable radicals have the advantage that their concentrations are readily and directly measurable. Among them a stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was investigated as a reactive hydrogen acceptor and further found to be useful for the antioxidant determination. Since then DPPH has been mainly used to examine radical scavenging activity of antioxidative vitamins and po...

متن کامل

Chem. Pharm. Bull. 53(2) 153—163 (2005)

derivatives (Fig. 1) that showed in vitro and in vivo antitumor activity. These compounds were also found to inhibit tubulin polymerization as a mechanism of their action in cells. To investigate the possibilities for modification of this scaffold, we divided the moieties into five parts from A to E as shown in Fig. 1. So far, it has been reported that the length of the three carbon chain on mo...

متن کامل

Chem. Pharm. Bull. 53(2) 260—262 (2005)

The size distribution of new vesicles formed after addition of oleate in different forms to preformed egg yolk phosphatidylcholine (EggPC) vesicles was studied by gel exclusion chromatography. The addition of oleate to preformed vesicles resulted in the formation of new small vesicles. Fission of preformed vesicles incorporated by oleate and partial solubilization of the vesicles by addition of...

متن کامل

Chem. Pharm. Bull. 53(3) 339—341 (2005)

tural elucidation of eighteen new and sixteen known acyl glucoses having caffeoyl, coumaroyl and hexahydroxydiphenoyl groups from Balanophora japonica M. (Balanophoraceae) collected in Nagasaki Perfecture, Japan which was used as an antipyretic, an antitode and a hemostatic medicine in China. In a continuation of our studies on the chemical constituents of medicinal parasitic plants, we further...

متن کامل

Chem. Pharm. Bull. 53(7) 783—787 (2005)

grown in western North America, we have identified a number of various types of phenolic compounds (nine phenylpropanoids, six neolignans, and seven flavonoids) in their glycoside form from the aerial parts of Juniperus communis var. depressa (Cupressaceae) and those results were reported in our previous papers. Upon continued chemical investigation of the same plant material, a new monoterpene...

متن کامل

Antiinflammatory Constituents of Teramnus labialis

1. Alagarsamy, V., Raja Salomon, V., Vanikavitha, G., Paluchamy, V., Ravichandran, M., Arnold Sujin, A., Thangathirupathy, A., Amuthalakshmi, S. and Revathi R., Biol. Pharm. Bull., 2002, 25, 1432. 2. Alagarsamy, V., Muthukumar, V., Pavalarani, N., Vasanthanathan, P. and Revathi R., Biol. Pharm. Bull., 2003, 26(4), 557. 3. Chaurasia, M.R. and Sharma, S.K., Arch. Pharm., 1982, 315, 377. 4. Manabu...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2005